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Cover picture: Depletion of phosphatidylinositol 4,5-bisphosphate alters epithelial morphology and actin distribution. Intestinal epithelial cells (IEC18) were microinjected with cDNAs encoding SigD, a bacterial phosphoinositide phosphatase and PLCδ-PH-GFP (green), a probe for phosphatidylinositol 4,5-bisphosphate. Samples were imaged by confocal microscopy at early (2 h; top) and late (5–6 h; bottom) stages after microinjection and stained for F-actin (red). X-Y scans (left and center) and X-Z reconstructions (right) are shown (see article by Mason et al., 267–283).
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