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Cover picture: Imaging Ca2+ flux through individual ion channels by total internal reflection fluorescence microscopy (TIRFM). Top schematic illustrates the optical "sectioning" of a Ca2+ microdomain around a single channel by the evanescent wave formed using through-the-lens TIRFM. Bottom panel shows several single-channel Ca2+ transients arising through openings of nicotinic receptors expressed in the membrane of a Xenopus oocyte (see article by Demuro and Parker, 179-192).
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