|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 98, 1105-1125, Copyright © 1991 by The Rockefeller University Press
ARTICLES |
MA Andrews, DW Maughan, TM Nosek and RE Godt
Department of Physiology & Endocrinology, Medical College of Georgia, Augusta 30912-3000.
We used single fibers from rabbit psoas muscle, chemically skinned with Triton X-100 nonionic detergent, to determine the salts best suited for adjusting ionic strength of bathing solutions for skinned fibers. As criteria we measured maximal calcium-activated force (Fmax), fiber swelling estimated optically, and protein extraction from single fibers determined by polyacrylamide gel electrophoresis with ultrasensitive silver staining. All things considered, the best uni-univalent salt was potassium methanesulfonate, while a number of uni-divalent potassium salts of phosphocreatine, hexamethylenediamine N,N,N',N'-tetraacetic acid, sulfate, and succinate were equally acceptable. Using these salts, we determined that changes in Fmax correlated best with variations of ionic strength (1/2 sigma ci z2i, where ci is the concentration of ion i, and zi is its valence) rather than ionic equivalents (1/2 sigma ci magnitude of zi). Our data indicate that increased ionic strength per sc decreases Fmax, probably by destabilizing the cross-bridge structure in addition to increasing electrostatic shielding of actomyosin interactions.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. A. Niederer, P. J. Hunter, and N. P. Smith A Quantitative Analysis of Cardiac Myocyte Relaxation: A Simulation Study Biophys. J., March 1, 2006; 90(5): 1697 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. I. Gokina, K. M. Park, K. McElroy-Yaggy, and G. Osol Effects of Rho kinase inhibition on cerebral artery myogenic tone and reactivity J Appl Physiol, May 1, 2005; 98(5): 1940 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Posterino and M. W. Fryer Effects of high myoplasmic L-lactate concentration on E-C coupling in mammalian skeletal muscle J Appl Physiol, August 1, 2000; 89(2): 517 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. I. Gokina, H. J. Knot, M. T. Nelson, and G. Osol Increased Ca2+ sensitivity as a key mechanism of PKC-induced constriction in pressurized cerebral arteries Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H1178 - H1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Befroy, T. Powell, G. K. Radda, and K. Clarke Osmotic shock: modulation of contractile function, pHi, and ischemic damage in perfused guinea pig heart Am J Physiol Heart Circ Physiol, April 1, 1999; 276(4): H1236 - H1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. I. Gokina and G. Osol Temperature and protein kinase C modulate myofilament Ca2+ sensitivity in pressurized rat cerebral arteries Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H1920 - H1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. LANG, G. L. BUSCH, M. RITTER, H. VOLKL, S. WALDEGGER, E. GULBINS, and D. HAUSSINGER Functional Significance of Cell Volume Regulatory Mechanisms Physiol Rev, January 1, 1998; 78(1): 247 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Blanchard, K. Iizuka, M. Christe, D. A. Conner, A. Geisterfer-Lowrance, F. J. Schoen, D. W. Maughan, C. E. Seidman, and J.G. Seidman Targeted Ablation of the Murine {alpha}-Tropomyosin Gene Circ. Res., December 19, 1997; 81(6): 1005 - 1010. [Abstract] [Full Text]
|
|
|
|
|
|
G.-R. Li, M. Zhang, L. S. Satin, and C. M. Baumgarten Biphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytes Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1270 - H1277. [Abstract] [Full Text] [PDF]
|
|
|
|
