The Journal of General Physiology, Vol 85, 291-320, Copyright © 1985 by The Rockefeller University Press

ARTICLES

Simulated calcium current can both cause calcium loading in and trigger calcium release from the sarcoplasmic reticulum of a skinned canine cardiac Purkinje cell

A Fabiato

Skinned canine cardiac Purkinje cells were stimulated by regularly repeated microinjection-aspiration sequences that were programmed to simulate the fast initial component of the transsarcolemmal Ca2+ current and the subsequent slow component corresponding to noninactivating Ca2+ channels. The simulated fast component triggered a tension transient through Ca2+-induced release of Ca2+ from the sarcoplasmic reticulum (SR). The simulated slow component did not affect the tension transient during which it was first introduced but it potentiated the subsequent transients. The potentiation was not observed when the SR function had been destroyed by detergent. The potentiation decreased progressively when the slow component was separated by an increasing time interval from the fast component. The potentiation was progressive over several beats under conditions that decreased the rate of Ca2+ accumulation into the SR (deletion of calmodulin from the solutions; a decrease of the temperature from 22 to 12 degrees C). In the presence of a slow component, an increase of frequency caused a positive staircase, and the introduction of an extrasystole caused a postextrasystolic potentiation. There was a negative staircase and no postextrasystolic potentiation in the absence of a slow component. These results can be explained by a time- and Ca2+- dependent functional separation of the release and accumulation processes of the SR, rather than by Ca2+ circulation between anatomically distinct loading and release compartments. The fast initial component of transsarcolemmal Ca2+ current would trigger Ca2+ release, whereas the slow component would load the SR with an amount of Ca2+ available for release during the subsequent tension transients.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. R. Laver and B. N. Honen Luminal Mg2+, A Key Factor Controlling RYR2-mediated Ca2+ Release: Cytoplasmic and Luminal Regulation Modeled in a Tetrameric Channel J. Gen. Physiol., October 1, 2008; 132(4): 429 - 446. [Abstract] [Full Text] [PDF]

				H. Cheng and W. J. Lederer Calcium Sparks Physiol Rev, October 1, 2008; 88(4): 1491 - 1545. [Abstract] [Full Text] [PDF]

				R. H. Shutt and S. E. Howlett Hypothermia increases the gain of excitation-contraction coupling in guinea pig ventricular myocytes Am J Physiol Cell Physiol, September 1, 2008; 295(3): C692 - C700. [Abstract] [Full Text] [PDF]

				X. Chen, X. Zhang, D. M. Harris, V. Piacentino III, R. M. Berretta, K. B. Margulies, and S. R. Houser Reduced effects of BAY K 8644 on L-type Ca2+ current in failing human cardiac myocytes are related to abnormal adrenergic regulation Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2257 - H2267. [Abstract] [Full Text] [PDF]

				V. A. Maltsev and E. G. Lakatta Dynamic interactions of an intracellular Ca2+ clock and membrane ion channel clock underlie robust initiation and regulation of cardiac pacemaker function Cardiovasc Res, January 18, 2008; (2008) cvm058v3. [Abstract] [Full Text] [PDF]

				C. Orchard and F. Brette t-tubules and sarcoplasmic reticulum function in cardiac ventricular myocytes Cardiovasc Res, January 15, 2008; 77(2): 237 - 244. [Abstract] [Full Text] [PDF]

				S. Hotta, K. Morimura, S. Ohya, K. Muraki, H. Takeshima, and Y. Imaizumi Ryanodine receptor type 2 deficiency changes excitation-contraction coupling and membrane potential in urinary bladder smooth muscle J. Physiol., July 15, 2007; 582(2): 489 - 506. [Abstract] [Full Text] [PDF]

				N. Kapur and K. Banach Inositol-1,4,5-trisphosphate-mediated spontaneous activity in mouse embryonic stem cell-derived cardiomyocytes J. Physiol., June 15, 2007; 581(3): 1113 - 1127. [Abstract] [Full Text] [PDF]

				F. Brette and C. Orchard Resurgence of Cardiac T-Tubule Research Physiology, June 1, 2007; 22(3): 167 - 173. [Abstract] [Full Text] [PDF]

				L. M. Livshitz and Y. Rudy Regulation of Ca2+ and electrical alternans in cardiac myocytes: role of CAMKII and repolarizing currents Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2854 - H2866. [Abstract] [Full Text] [PDF]

				S. A. Grandy and S. E. Howlett Cardiac excitation-contraction coupling is altered in myocytes from aged male mice but not in cells from aged female mice Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2362 - H2370. [Abstract] [Full Text] [PDF]

				R. H. Shutt, G. R. Ferrier, and S. E. Howlett Increases in diastolic [Ca2+] can contribute to positive inotropy in guinea pig ventricular myocytes in the absence of changes in amplitudes of Ca2+ transients Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1623 - H1634. [Abstract] [Full Text] [PDF]

				F. Brette and C. H. Orchard No Apparent Requirement for Neuronal Sodium Channels in Excitation-Contraction Coupling in Rat Ventricular Myocytes Circ. Res., March 17, 2006; 98(5): 667 - 674. [Abstract] [Full Text] [PDF]

				F. Brette, L. Salle, and C. H. Orchard Quantification of Calcium Entry at the T-Tubules and Surface Membrane in Rat Ventricular Myocytes Biophys. J., January 1, 2006; 90(1): 381 - 389. [Abstract] [Full Text] [PDF]

				N. Lindegger and E. Niggli Paradoxical SR Ca2+ release in guinea-pig cardiac myocytes after {beta}-adrenergic stimulation revealed by two-photon photolysis of caged Ca2+ J. Physiol., June 15, 2005; 565(3): 801 - 813. [Abstract] [Full Text] [PDF]

				M. R Fowler, R. S Dobson, C. H Orchard, and S. M Harrison Functional consequences of detubulation of isolated rat ventricular myocytes Cardiovasc Res, June 1, 2004; 62(3): 529 - 537. [Abstract] [Full Text] [PDF]

				A. G. KLEBER and Y. RUDY Basic Mechanisms of Cardiac Impulse Propagation and Associated Arrhythmias Physiol Rev, April 1, 2004; 84(2): 431 - 488. [Abstract] [Full Text] [PDF]

				D. Yang, L.-S. Song, W.-Z. Zhu, K. Chakir, W. Wang, C. Wu, Y. Wang, R.-P. Xiao, S.R. W. Chen, and H. Cheng Calmodulin Regulation of Excitation-Contraction Coupling in Cardiac Myocytes Circ. Res., April 4, 2003; 92(6): 659 - 667. [Abstract] [Full Text] [PDF]

				C. E. Sears, S. M. Bryant, E. A. Ashley, C. A. Lygate, S. Rakovic, H. L. Wallis, S. Neubauer, D. A. Terrar, and B. Casadei Cardiac Neuronal Nitric Oxide Synthase Isoform Regulates Myocardial Contraction and Calcium Handling Circ. Res., March 21, 2003; 92 (5): e52 - e59. [Abstract] [Full Text] [PDF]

				K. F Frank, B. Bolck, E. Erdmann, and R. H.G Schwinger Sarcoplasmic reticulum Ca2+-ATPase modulates cardiac contraction and relaxation Cardiovasc Res, January 1, 2003; 57(1): 20 - 27. [Abstract] [Full Text] [PDF]

				F. Protasi, C. Paolini, J. Nakai, K. G. Beam, C. Franzini-Armstrong, and P. D. Allen Multiple Regions of RyR1 Mediate Functional and Structural Interactions with alpha 1S-Dihydropyridine Receptors in Skeletal Muscle Biophys. J., December 1, 2002; 83(6): 3230 - 3244. [Abstract] [Full Text] [PDF]

				S.-S. Zhou, Z. Gao, L. Dong, Y.-F. Ding, X.-D. Zhang, Y.-M. Wang, J.-M. Pei, F. Gao, and X.-L. Ma Anion channels influence ECC by modulating L-type Ca2+ channel in ventricular myocytes J Appl Physiol, November 1, 2002; 93(5): 1660 - 1668. [Abstract] [Full Text] [PDF]

				V. Sharma and L. Tung Effects of uniform electric fields on intracellular calcium transients in single cardiac cells Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H72 - H79. [Abstract] [Full Text] [PDF]

				G. R. Ferrier and S. E. Howlett Cardiac excitation-contraction coupling: role of membrane potential in regulation of contraction Am J Physiol Heart Circ Physiol, May 1, 2001; 280(5): H1928 - H1944. [Abstract] [Full Text] [PDF]

				B. G. Kerfant, G. Vassort, and A. M. Gomez Microtubule Disruption by Colchicine Reversibly Enhances Calcium Signaling in Intact Rat Cardiac Myocytes Circ. Res., April 13, 2001; 88 (7): e59 - e65. [Abstract] [Full Text] [PDF]

				A. W. Trafford, M. E. Diaz, and D. A. Eisner Coordinated Control of Cell Ca2+ Loading and Triggered Release From the Sarcoplasmic Reticulum Underlies the Rapid Inotropic Response to Increased L-Type Ca2+ Current Circ. Res., February 2, 2001; 88(2): 195 - 201. [Abstract] [Full Text] [PDF]

				N. Matsuda, K. G. Morgan, and F. W. Sellke Effects of pinacidil on coronary Ca2+-myosin phosphorylation in cold potassium cardioplegia model Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H882 - H888. [Abstract] [Full Text] [PDF]

				J.-B. Shen, B. Jiang, and A. J. Pappano Comparison of L-Type Calcium Channel Blockade by Nifedipine and/or Cadmium in Guinea Pig Ventricular Myocytes J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 562 - 570. [Abstract] [Full Text]

				S. Barrere-Lemaire, C. Piot, F. Leclercq, J. Nargeot, and S. Richard Facilitation of L-type calcium currents by diastolic depolarization in cardiac cells: impairment in heart failure Cardiovasc Res, August 1, 2000; 47(2): 336 - 349. [Abstract] [Full Text] [PDF]

				J. J. Rice, M. S. Jafri, and R. L. Winslow Modeling short-term interval-force relations in cardiac muscle Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H913 - H931. [Abstract] [Full Text] [PDF]

				Y. Qu, A. Ghatpande, N. El-Sherif, and M. Boutjdir Gene expression of Na+/Ca2+ exchanger during development in human heart Cardiovasc Res, March 1, 2000; 45(4): 866 - 873. [Abstract] [Full Text] [PDF]

				H. Shiels, M Vornanen, and A. Farrell Temperature-dependence of L-type Ca(2+) channel current in atrial myocytes from rainbow trout J. Exp. Biol., January 9, 2000; 203(18): 2771 - 2780. [Abstract] [PDF]

				I. A Hobai and A. J Levi Coming full circle: Membrane potential, sarcolemmal calcium influx and excitation-contraction coupling in heart muscle Cardiovasc Res, December 1, 1999; 44(3): 477 - 487. [Abstract] [Full Text] [PDF]

				N. Matsuda, K. G. Morgan, and F. W. Sellke PRECONDITIONING IMPROVES CARDIOPLEGIA-RELATED CORONARY MICROVASCULAR SMOOTH MUSCLE HYPERCONTRACTILITY: ROLE OF KATP CHANNELS J. Thorac. Cardiovasc. Surg., September 1, 1999; 118(3): 438 - 445. [Abstract] [Full Text] [PDF]

				M. Kawai, M. Hussain, and C. H. Orchard Excitation-contraction coupling in rat ventricular myocytes after formamide-induced detubulation Am J Physiol Heart Circ Physiol, August 1, 1999; 277(2): H603 - H609. [Abstract] [Full Text] [PDF]

				N. Matsuda, M. Tofukuji, K. G. Morgan, and F. W. Sellke Coronary microvascular protection with Mg2+: effects on intracellular calcium regulation and vascular function Am J Physiol Heart Circ Physiol, April 1, 1999; 276(4): H1124 - H1130. [Abstract] [Full Text] [PDF]

				S. G. Priori, J. Barhanin, R. N. W. Hauer, W. Haverkamp, H. J. Jongsma, A. G. Kleber, W. J. McKenna, D. M. Roden, Y. Rudy, K. Schwartz, et al. Genetic and Molecular Basis of Cardiac Arrhythmias: Impact on Clinical Management Part III Circulation, February 9, 1999; 99(5): 674 - 681. [Full Text] [PDF]

				S.G. Priori, J. Barhanin, R.N.W. Hauer, W. Haverkamp, H.J. Jongsma, A.G. Kleber, W.J. McKenna, D.M. Roden, Y. Rudy, K. Schwartz, et al. Genetic and molecular basis of cardiac arrhythmias: Impact on clinical management Eur. Heart J., February 1, 1999; 20(3): 174 - 195. [PDF]

				J. S. K. Sham, L.-S. Song, Y. Chen, L.-H. Deng, M. D. Stern, E. G. Lakatta, and H. Cheng Termination of Ca2+ release by a local inactivation of ryanodine receptors in cardiac myocytes PNAS, December 8, 1998; 95(25): 15096 - 15101. [Abstract] [Full Text] [PDF]

				H. Sun, R. Gaspo, N. Leblanc, and S. Nattel Cellular Mechanisms of Atrial Contractile Dysfunction Caused by Sustained Atrial Tachycardia Circulation, August 18, 1998; 98(7): 719 - 727. [Abstract] [Full Text] [PDF]

				M. K. Slodzinski and M. P. Blaustein Na+/Ca2+ exchange in neonatal rat heart cells: antisense inhibition and protein half-life Am J Physiol Cell Physiol, August 1, 1998; 275(2): C459 - C467. [Abstract] [Full Text] [PDF]

				D.A Eisner, A.W Trafford, M.E Dnaz, C.L Overend, and S.C O'Neill The control of Ca release from the cardiac sarcoplasmic reticulum: regulation versus autoregulation Cardiovasc Res, June 1, 1998; 38(3): 589 - 604. [Abstract] [Full Text] [PDF]

				L. F. Santana, A. M. Gómez, and W. J. Lederer Ca2+ Flux Through Promiscuous Cardiac Na+ Channels: Slip-Mode Conductance Science, February 13, 1998; 279(5353): 1027 - 1033. [Abstract] [Full Text]

				J. A Mattiello, K. B Margulies, V. Jeevanandam, and S. R Houser Contribution of reverse-mode sodium-calcium exchange to contractions in failing human left ventricular myocytes Cardiovasc Res, February 1, 1998; 37(2): 424 - 431. [Abstract] [Full Text] [PDF]

				K. R. Sipido, M. Maes, and F. Van de Werf Low Efficiency of Ca2+ Entry Through the Na+-Ca2+ Exchanger as Trigger for Ca2+ Release From the Sarcoplasmic Reticulum : A Comparison Between L-Type Ca2+ Current and Reverse-Mode Na+-Ca2+ Exchange Circ. Res., December 19, 1997; 81(6): 1034 - 1044. [Abstract] [Full Text]

				B. H. Dorman, L. Hebbar, R. B. Hinton, R. C. Roy, and F. G. Spinale Preservation of Myocyte Contractile Function After Hypothermic Cardioplegic Arrest by Activation of ATP-Sensitive Potassium Channels Circulation, October 7, 1997; 96(7): 2376 - 2384. [Abstract] [Full Text]

				A.M Evans and M.B Cannell The role of L-type Ca2+ current and Na+ current-stimulated Na/Ca exchange in triggering SR calcium release in guinea-pig cardiac ventricular myocytes Cardiovasc Res, August 1, 1997; 35(2): 294 - 302. [Abstract] [Full Text] [PDF]

				A. M. Gómez, H. H. Valdivia, H. Cheng, M. R. Lederer, L. F. Santana, M. B. Cannell, S. A. McCune, R. A. Altschuld, and W. J. Lederer Defective Excitation-Contraction Coupling in Experimental Cardiac Hypertrophy and Heart Failure Science, May 2, 1997; 276(5313): 800 - 806. [Abstract] [Full Text]

				M. Vornanen and N. Shepherd Restitution of contractility in single ventricular myocytes of guinea pig heart Cardiovasc Res, March 1, 1997; 33(3): 611 - 622. [Abstract] [PDF]

				J. R. Lopez, R. Jahangir, A. Jahangir, W. K. Shen, and A. Terzic POTASSIUM CHANNEL OPENERS PREVENT POTASSIUM-INDUCED CALCIUM LOADING OF CARDIAC CELLS: POSSIBLE IMPLICATIONS IN CARDIOPLEGIA J. Thorac. Cardiovasc. Surg., September 1, 1996; 112(3): 820 - 831. [Abstract] [Full Text]

				C.J. Grantham and M.B. Cannell Ca2+ Influx During the Cardiac Action Potential in Guinea Pig Ventricular Myocytes Circ. Res., August 1, 1996; 79(2): 194 - 200. [Abstract] [Full Text]

				L.F. Santana, H. Cheng, A.M. Gomez, M.B. Cannell, and W.J. Lederer Relation Between the Sarcolemmal Ca2+ Current and Ca2+ Sparks and Local Control Theories for Cardiac Excitation-Contraction Coupling Circ. Res., January 1, 1996; 78(1): 166 - 171. [Abstract] [Full Text]

				K. Ono, N. Gondo, M. Arita, H. A. Fozzard, T. Hadama, and Y. Uchida University of Wisconsin Solution Preserves Myocardial Calcium Current Response to Isoproterenol in Isolated Canine Ventricular Myocytes Circulation, November 1, 1995; 92(9): 452 - 457. [Abstract] [Full Text]

				C. M. N. Terracciano, R. U. Naqvi, and K. T. MacLeod Effects of Rest Interval on the Release of Calcium From the Sarcoplasmic Reticulum in Isolated Guinea Pig Ventricular Myocytes Circ. Res., August 1, 1995; 77(2): 354 - 360. [Abstract] [Full Text]

				R. A. Bouchard, R. B. Clark, and W. R. Giles Effects of Action Potential Duration on Excitation-Contraction Coupling in Rat Ventricular Myocytes : Action Potential Voltage-Clamp Measurements Circ. Res., May 1, 1995; 76(5): 790 - 801. [Abstract] [Full Text]

				H Cheng, M Fill, H Valdivia, and W. Lederer Models of Ca2+ release channel adaptation Science, March 31, 1995; 267(5206): 2009 - 2010. [PDF]

				H. Cheng, M. B. Cannell, and W. J. Lederer Partial Inhibition of Ca2+ Current by Methoxyverapamil (D600) Reveals Spatial Nonuniformities in [Ca2+]i During Excitation-Contraction Coupling in Cardiac Myocytes Circ. Res., February 1, 1995; 76(2): 236 - 241. [Abstract] [Full Text]

				J. Sham, L Cleemann, and M Morad Gating of the cardiac Ca2+ release channel: the role of Na+ current and Na(+)-Ca2+ exchange Science, February 14, 1992; 255(5046): 850 - 853. [Abstract] [PDF]