|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 84, 505-534, Copyright © 1984 by The Rockefeller University Press
ARTICLES |
R Horn and CA Vandenberg
Single channel currents were obtained from voltage-activated sodium channels in outside-out patches of tissue-cultured GH3 cells, a clonal line from rat pituitary gland. In membrane patches where the probability of overlapping openings was low, the open time histograms were well fit by a single exponential. Most analysis was done on a patch with exactly one channel. We found no evidence for multiple open states at -25 and -40 mV, since open times, burst durations, and autocorrelation functions were time independent. Amplitude histograms showed no evidence of multiple conductance levels. We fit the gating with 25 different time-homogeneous Markov chain models having up to five states, using a maximum likelihood procedure to estimate the rate constants. For selected models, this procedure yielded excellent predictions for open time, closed time, and first latency density functions, as well as the probability of the channel being open after a step depolarization, the burst duration distribution, autocorrelation, and the distribution of number of openings per record. The models were compared statistically using likelihood ratio tests and Akaike's information criterion. Acceptable models allowed inactivation from closed states, as well as from the open state. Among the models eliminated as unacceptable by this survey were the Hodgkin-Huxley model and any model requiring a channel to open before inactivating.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Shelley and K. L. Magleby Linking Exponential Components to Kinetic States in Markov Models for Single-Channel Gating J. Gen. Physiol., July 28, 2008; 132(2): 295 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Groome, M. C. Dice, E. Fujimoto, and P. C. Ruben Charge Immobilization of Skeletal Muscle Na+ Channels: Role of Residues in the Inactivation Linker Biophys. J., September 1, 2007; 93(5): 1519 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Clancy, Z. I. Zhu, and Y. Rudy Pharmacogenetics and anti-arrhythmic drug therapy: a theoretical investigation Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H66 - H75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Kahlig, S. N. Misra, and A. L. George Jr Impaired Inactivation Gate Stabilization Predicts Increased Persistent Current for an Epilepsy-Associated SCN1A Mutation J. Neurosci., October 25, 2006; 26(43): 10958 - 10966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Csanady Statistical Evaluation of Ion-Channel Gating Models Based on Distributions of Log-Likelihood Ratios Biophys. J., May 15, 2006; 90(10): 3523 - 3545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. H. Kwan, D. Fedida, and S. J. Kehl Single Channel Analysis Reveals Different Modes of Kv1.5 Gating Behavior Regulated by Changes of External pH Biophys. J., February 15, 2006; 90(4): 1212 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ulbricht Sodium Channel Inactivation: Molecular Determinants and Modulation Physiol Rev, October 1, 2005; 85(4): 1271 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Clancy and R. S. Kass Theoretical Investigation of the Neuronal Na+ Channel SCN1A: Abnormal Gating and Epilepsy Biophys. J., April 1, 2004; 86(4): 2606 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Adair Noise and stochastic resonance in voltage-gated ion channels PNAS, October 14, 2003; 100(21): 12099 - 12104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baruscotti, D. DiFrancesco, and R. B. Robinson Na+ current contribution to the diastolic depolarization in newborn rabbit SA node cells Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2303 - H2309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Legendre Voltage Dependence of the Glycine Receptor-Channel Kinetics in the Zebrafish Hindbrain J Neurophysiol, November 1, 1999; 82(5): 2120 - 2129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kellenberger, T. Scheuer, and W. A. Catterall Movement of the Na+ Channel Inactivation Gate during Inactivation J. Biol. Chem., November 29, 1996; 271(48): 30971 - 30979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Bennett, C. Valenzuela, L.-Q. Chen, and R. G. Kallen On the Molecular Nature of the Lidocaine Receptor of Cardiac Na+ Channels : Modification of Block by Alterations in the {alpha}-Subunit III-IV Interdomain Circ. Res., September 1, 1995; 77(3): 584 - 592. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Catterall Structure and function of voltage-sensitive ion channels Science, October 7, 1988; 242(4875): 50 - 61. [Abstract] [PDF]
|
|
|
|
|
|
R. Weiss and R Horn Functional differences between two classes of sodium channels in developing rat skeletal muscle Science, July 18, 1986; 233(4761): 361 - 364. [Abstract] [PDF]
|
|
|
|
