|
|
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Journal of General Physiology, Vol 82, 25-45, Copyright © 1983 by The Rockefeller University Press
ARTICLES |
W Hanke and C Miller
Single-channel fluctuations of a chloride-specific channel from Torpedo californica electroplax were studied with high current and time resolution. Channels were incorporated into virtually solvent-free planar bilayer membranes formed from phospholipid monolayers, and the substructure of the open channel was analyzed. The single channel displays three well-defined substates of conductances 0, 10, and 20 pS in 200 mM Cl-. These three substates are interpreted in terms of a dimeric channel complex composed of two identical "protochannels" gating independently in parallel on a time scale of milliseconds, but coupled together by a bursting process on a time scale of seconds. The probability of forming an open protochannel is voltage dependent and is increased strongly as aqueous pH is lowered. Variations of pH are effective only on the same side of the bilayer as the addition of electroplax vesicles. The dependence of single-channel kinetics on pH and voltage lead to a minimal four-state model in which both open and closed states can be protonated on a residue that changes its pK from 6 to 9 upon opening of the protochannel.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Zifarelli, A. R. Murgia, P. Soliani, and M. Pusch Intracellular Proton Regulation of ClC-0 J. Gen. Physiol., July 1, 2008; 132(1): 185 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-Y. Chen and T.-C. Hwang CLC-0 and CFTR: Chloride Channels Evolved From Transporters Physiol Rev, April 1, 2008; 88(2): 351 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Engh, J. D. Faraldo-Gomez, and M. Maduke The Mechanism of Fast-Gate Opening in ClC-0 J. Gen. Physiol., September 24, 2007; 130(4): 335 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-Y. Tseng, B. Bennetts, and T.-Y. Chen Cytoplasmic ATP Inhibition of CLC-1 Is Enhanced by Low pH J. Gen. Physiol., July 30, 2007; 130(2): 217 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Denton, K. Nehrke, X. Yin, A. M. Beld, and K. Strange Altered gating and regulation of a carboxy-terminal ClC channel mutant expressed in the Caenorhabditis elegans oocyte Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1109 - C1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-D. Zhang, Y. Li, W.-P. Yu, and T.-Y. Chen Roles of K149, G352, and H401 in the Channel Functions of ClC-0: Testing the Predictions from Theoretical Calculations J. Gen. Physiol., March 27, 2006; 127(4): 435 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Traverso, G. Zifarelli, R. Aiello, and M. Pusch Proton Sensing of CLC-0 Mutant E166D J. Gen. Physiol., December 27, 2005; 127(1): 51 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. de Santiago, K. Nehrke, and J. Arreola Quantitative Analysis of the Voltage-dependent Gating of Mouse Parotid ClC-2 Chloride Channel J. Gen. Physiol., November 28, 2005; 126(6): 591 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Czajkowsky, H. Iwamoto, G. Szabo, T. L. Cover, and Z. Shao Mimicry of a Host Anion Channel by a Helicobacter pylori Pore-Forming Toxin Biophys. J., November 1, 2005; 89(5): 3093 - 3101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matulef and M. Maduke Side-Dependent Inhibition of a Prokaryotic ClC by DIDS Biophys. J., September 1, 2005; 89(3): 1721 - 1730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, W.-P. Yu, C.-W. Lin, and T.-Y. Chen Oxidation and Reduction Control of the Inactivation Gating of Torpedo ClC-0 Chloride Channels Biophys. J., June 1, 2005; 88(6): 3936 - 3945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Engh and M. Maduke Cysteine Accessibility in ClC-0 Supports Conservation of the ClC Intracellular Vestibule J. Gen. Physiol., May 31, 2005; 125(6): 601 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Bostick and M. L. Berkowitz Exterior Site Occupancy Infers Chloride-Induced Proton Gating in a Prokaryotic Homolog of the ClC Chloride Channel Biophys. J., September 1, 2004; 87(3): 1686 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Accardi, L. Kolmakova-Partensky, C. Williams, and C. Miller Ionic Currents Mediated by a Prokaryotic Homologue of CLC Cl- Channels J. Gen. Physiol., January 26, 2004; 123(2): 109 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cai, T. S. Scott-Ward, and D. N. Sheppard Voltage-dependent Gating of the Cystic Fibrosis Transmembrane Conductance Regulator Cl- Channel J. Gen. Physiol., October 27, 2003; 122(5): 605 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Chen, M.-F. Chen, and C.-W. Lin Electrostatic Control and Chloride Regulation of the Fast Gating of ClC-0 Chloride Channels J. Gen. Physiol., October 27, 2003; 122(5): 641 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Traverso, L. Elia, and M. Pusch Gating Competence of Constitutively Open CLC-0 Mutants Revealed by the Interaction with a Small Organic Inhibitor J. Gen. Physiol., August 25, 2003; 122(3): 295 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Accardi and M. Pusch Conformational Changes in the Pore of CLC-0 J. Gen. Physiol., August 25, 2003; 122(3): 277 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-F. Chen and T.-Y. Chen Side-chain Charge Effects and Conductance Determinants in the Pore of ClC-0 Chloride Channels J. Gen. Physiol., July 28, 2003; 122(2): 133 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Lin and T.-Y. Chen Probing the Pore of ClC-0 by Substituted Cysteine Accessibility Method Using Methane Thiosulfonate Reagents J. Gen. Physiol., July 28, 2003; 122(2): 147 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Cooper and P. Fong Relationship between intracellular pH and chloride in Xenopus oocytes expressing the chloride channel ClC-0 Am J Physiol Cell Physiol, February 1, 2003; 284(2): C331 - C338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Warnstedt, C. Sun, B. Poser, M. J. Escriva, L. Tranebjarg, T. Torbergsen, M. van Ghelue, and C. Fahlke The Myotonia Congenita Mutation A331T Confers a Novel Hyperpolarization-Activated Gate to the Muscle Chloride Channel ClC-1 J. Neurosci., September 1, 2002; 22(17): 7462 - 7470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Aromataris, G. Y. Rychkov, B. Bennetts, B. P. Hughes, A. H. Bretag, and M. L. Roberts Fast and Slow Gating of CLC-1: Differential Effects of 2-(4-Chlorophenoxy) Propionic Acid and Dominant Negative Mutations Mol. Pharmacol., July 1, 2001; 60(1): 200 - 208. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. WALDEGGER and T. J. JENTSCH From Tonus to Tonicity: Physiology of CLC Chloride Channels J. Am. Soc. Nephrol., July 1, 2000; 11(7): 1331 - 1339. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Chalfant, J. S. Denton, B. K. Berdiev, I. I. Ismailov, D. J. Benos, and B. A. Stanton Intracellular H+ regulates the alpha -subunit of ENaC, the epithelial Na+ channel Am J Physiol Cell Physiol, February 1, 1999; 276(2): C477 - C486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pleumsamran, M. L. Wolak, and D. Kim Inhibition of ATP-induced increase in muscarinic K+ current by trypsin, alkaline pH, and anions Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H751 - H759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fong, A. Rehfeldt, and T. J. Jentsch Determinants of slow gating in ClC-0, the voltage-gated chloride channel of Torpedo marmorata Am J Physiol Cell Physiol, April 1, 1998; 274(4): C966 - C973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Weinreich and T. J. Jentsch Pores Formed by Single Subunits in Mixed Dimers of Different CLC Chloride Channels J. Biol. Chem., January 19, 2001; 276(4): 2347 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
