|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 81, 773-784, Copyright © 1983 by The Rockefeller University Press
ARTICLES |
RE Taylor and F Bezanilla
The sodium and gating currents of the squid giant axon elicited by a depolarizing pulse are delayed, with little change in shape, as a result of a hyperpolarizing prepulse. The delays are almost completely saturated, at approximately 45 microseconds, for prepulses to -140 mV. At 8 degrees C they develop with time constants of between 60 and 180 microseconds for prepulses in the -130- to -150-mV range. There is a correlation between the extra charge moved during the gating current and the increase in the time delay of the sodium current as the magnitude of the hyperpolarizing prepulse is increased. These results strengthen the conclusion that the gating current is indeed closely associated with the process of sodium channel opening and provide information concerning the kinetics of the early steps, which are hidden in ionic current measurements. The main features of the gating and sodium current time shifts and the correlation between charge movement and time shifts are duplicated by a sequential six-state model for sodium activation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. V. Campos, B. Chanda, P. S.L. Beirao, and F. Bezanilla {beta}-Scorpion Toxin Modifies Gating Transitions in All Four Voltage Sensors of the Sodium Channel J. Gen. Physiol., August 27, 2007; 130(3): 257 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. A. Bannister, B. Chanda, F. Bezanilla, and D. M. Papazian Optical detection of rate-determining ion-modulated conformational changes of the ether-a-go-go K+ channel voltage sensor PNAS, December 20, 2005; 102(51): 18718 - 18723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. N. Filatov, M. J. Pinter, and M. M. Rich Resting Potential-dependent Regulation of the Voltage Sensitivity of Sodium Channel Gating in Rat Skeletal Muscle In Vivo J. Gen. Physiol., July 25, 2005; 126(2): 161 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuzmenkin, F. Bezanilla, and A. M. Correa Gating of the Bacterial Sodium Channel, NaChBac: Voltage-dependent Charge Movement and Gating Currents J. Gen. Physiol., September 27, 2004; 124(4): 349 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bezanilla The Voltage Sensor in Voltage-Dependent Ion Channels Physiol Rev, April 1, 2000; 80(2): 555 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Bezanilla, E Perozo, D. Papazian, and E Stefani Molecular basis of gating charge immobilization in Shaker potassium channels Science, November 1, 1991; 254(5032): 679 - 683. [Abstract] [PDF]
|
|
|
|
