The Journal of General Physiology
CrossRef
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1436K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsacopoulos, M.
Right arrow Articles by Borsellino, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsacopoulos, M.
Right arrow Articles by Borsellino, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Journal of General Physiology, Vol 77, 601-628, Copyright © 1981 by The Rockefeller University Press

ARTICLES

Diffusion and consumption of oxygen in the superfused retina of the drone (Apis mellifera) in darkness

M Tsacopoulos, S Poitry and A Borsellino

Double-barreled O2 microelectrodes were used to study O2 diffusion and consumption in the superfused drone (Apis mellifera) retina in darkness at 22 degrees C. Po2 was measured at different sites in the bath and retinas. It was found that diffusion was essentially in one dimension and that the rate of O2 consumption (Q) was practically constant (on the macroscale) down to Po2 s less than 20 mm Hg, a situation that greatly simplified the analysis. The value obtained for Q was 18 +/- 0.7 (SEM) microliter O2/cm3 tissue . min (n = 10), and Krogh's permeation coefficient (alpha D) was 3.24 +/- 0.18 (SEM) X 10(-5) ml O1/min . atm . cm (n = 10). Calculations indicate that only a small fraction of this Q in darkness is necessary for the energy requirements of the sodium pump. the diffusion coefficient (D) in the retina was measured by abruptly cutting off diffusion from the bath and analyzing the time-course of the fall in Po2 at the surface of the tissue. The mean value of D was 1.03 +/- 0.08 (SEM) X 10(-5) cm2/s (n = 10). From alpha D and D, the solubility coefficient alpha was calculated to be 54 +/- 4.0 (SEM) microliter O2 STP/cm3 . atm (n = 10), approximately 1.8 times that for water.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
J. A. Coles, J.-L. Martiel, and K. Laskowska
A glia-neuron alanine/ammonium shuttle is central to energy metabolism in bee retina
J. Physiol., April 15, 2008; 586(8): 2077 - 2091.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
I. K. Petropoulos, J.-A. C. Pournaras, J.-L. Munoz, and C. J. Pournaras
Effect of Carbogen Breathing and Acetazolamide on Optic Disc PO2
Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4139 - 4146.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Kukley, M. Schwan, B. B. Fredholm, and D. Dietrich
The Role of Extracellular Adenosine in Regulating Mossy Fiber Synaptic Plasticity
J. Neurosci., March 16, 2005; 25(11): 2832 - 2837.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J.-A. C. Pournaras, I. K. Petropoulos, J.-L. Munoz, and C. J. Pournaras
Experimental Retinal Vein Occlusion: Effect of Acetazolamide and Carbogen (95% O2/5% CO2) on Preretinal PO2
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3669 - 3677.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Agam, M. von Campenhausen, S. Levy, H. C. Ben-Ami, B. Cook, K. Kirschfeld, and B. Minke
Metabolic Stress Reversibly Activates the Drosophila Light-Sensitive Channels TRP and TRPL In Vivo
J. Neurosci., August 1, 2000; 20(15): 5748 - 5755.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents