|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 67, 325-341, Copyright © 1976 by The Rockefeller University Press
ARTICLES |
DB Rhoads, FB Waters and W Epstein
Analysis of K transport mutants indicates the existence of four separate K uptake systems in Escherichia coli K-12. A high affinity system called Kdp has a Km of 2 muM, and Vmax at 37 degrees C of 150 mumol/g min. This system is repressed by growth in high concentrations of K. Two constitutive systems, TrkA and TrkD, have Km's of 1.5 and 0.5 mM and Vmax's of 550 and 40 at 37 and 30 degrees C, respectively. Mutants lacking all three of these saturable systems take up K slowly by a process, called TrkF, whose rate of transport is linearly dependent on K concentration up to 105 mM. On the whole, each of these systems appears to function as an independent path for K uptake since the kinetics of uptake when two are present is the sum of each operating alone. This is not true for strains having both the TrkD and Kdp systems, where presence of the latter results in K uptake which saturates at a K concentration well below 0.1 mM. This result indicates some interaction between these systems so that uptake now has the affinity characteristic of the Kdp system. All transport systems are able to extrude Na during K uptake. The measurements of cell Na suggest that growing cells of E. coli have very low concentrations of Na, considerably lower than indicated by earlier studies.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Grabov Plant KT/KUP/HAK Potassium Transporters: Single Family - Multiple Functions Ann. Bot., June 1, 2007; 99(6): 1035 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-R. Lee, S.-H. Cho, M.-J. Yoon, A. Peterkofsky, and Y.-J. Seok Escherichia coli enzyme IIANtr regulates the K+ transporter TrkA PNAS, March 6, 2007; 104(10): 4124 - 4129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Santos, A. Lundby, C. Zazueta, and M. Montal Molecular Template for a Voltage Sensor in a Novel K+ Channel. I. Identification and Functional Characterization of KvLm, a Voltage-gated K+ Channel from Listeria monocytogenes J. Gen. Physiol., August 28, 2006; 128(3): 283 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Radchenko, K. Tanaka, R. Waditee, S. Oshimi, Y. Matsuzaki, M. Fukuhara, H. Kobayashi, T. Takabe, and T. Nakamura Potassium/Proton Antiport System of Escherichia coli J. Biol. Chem., July 21, 2006; 281(29): 19822 - 19829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kraegeloh, B. Amendt, and H. J. Kunte Potassium Transport in a Halophilic Member of the Bacteria Domain: Identification and Characterization of the K+ Uptake Systems TrkH and TrkI from Halomonas elongata DSM 2581T J. Bacteriol., February 1, 2005; 187(3): 1036 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Buurman, D. McLaggan, J. Naprstek, and W. Epstein Multiple Paths for Nonphysiological Transport of K+ in Escherichia coli J. Bacteriol., July 1, 2004; 186(13): 4238 - 4245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Benito, B. Garciadeblas, P. Schreier, and A. Rodriguez-Navarro Novel P-Type ATPases Mediate High-Affinity Potassium or Sodium Uptake in Fungi Eukaryot. Cell, April 1, 2004; 3(2): 359 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Chen, Y.-C. Chuang, C.-C. Chang, C.-L. Jeang, and M.-C. Chang A K+ Uptake Protein, TrkA, Is Required for Serum, Protamine, and Polymyxin B Resistance in Vibrio vulnificus Infect. Immun., February 1, 2004; 72(2): 629 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Batiza, M. M.-C. Kuo, K. Yoshimura, and C. Kung Gating the bacterial mechanosensitive channel MscL invivo PNAS, April 16, 2002; 99(8): 5643 - 5648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Woodbury, S. J.S. Hardy, and L. L. Randall Complex behavior in solution of homodimeric SecA Protein Sci., April 1, 2002; 11(4): 875 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jung, M. Krabusch, and K. Altendorf Cs+ Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K+ Concentration J. Bacteriol., June 15, 2001; 183(12): 3800 - 3803. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ga{beta}el, T. Mollenkamp, W. Puppe, and K. Altendorf The KdpF Subunit Is Part of the K+-translocating Kdp Complex of Escherichia coli and Is Responsible for Stabilization of the Complex in Vitro J. Biol. Chem., December 31, 1999; 274(53): 37901 - 37907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Steel, N. M. Matlola, and R. Anderson Inhibition of potassium transport and growth of mycobacteria exposed to clofazimine and B669 is associated with a calcium-independent increase in microbial phospholipase A2 activity J. Antimicrob. Chemother., August 1, 1999; 44(2): 209 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Malli and W. Epstein Expression of the Kdp ATPase Is Consistent with Regulation by Turgor Pressure J. Bacteriol., October 1, 1998; 180(19): 5102 - 5108. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ukai, H. Matsuzawa, K. Ito, M. Yamada, and A. Nishimura ftsE(Ts) Affects Translocation of K+-Pump Proteins into the Cytoplasmic Membrane of Escherichia coli J. Bacteriol., July 15, 1998; 180(14): 3663 - 3670. [Abstract] [Full Text]
|
|
|
|
|
|
T. Nakamura, R. Yuda, T. Unemoto, and E. P. Bakker KtrAB, a New Type of Bacterial K+-Uptake System from Vibrio alginolyticus J. Bacteriol., July 1, 1998; 180(13): 3491 - 3494. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Liang, C. H. Ko, T. Herman, and R. F. Gaber Trinucleotide Insertions, Deletions, and Point Mutations in Glucose Transporters Confer K+ Uptake in Saccharomyces cerevisiae Mol. Cell. Biol., February 1, 1998; 18(2): 926 - 935. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. J. Kim, J. M. Kwak, N. Uozumi, and J. I. Schroeder AtKUP1: An Arabidopsis Gene Encoding High-Affinity Potassium Transport Activity PLANT CELL, January 1, 1998; 10(1): 51 - 62. [Abstract] [Full Text]
|
|
|
|
|
|
W. Puppe, P. Zimmann, K. Jung, M. Lucassen, and K. Altendorf Characterization of Truncated Forms of the KdpD Protein, the Sensor Kinase of the K+-translocating Kdp System of Escherichia coli J. Biol. Chem., October 4, 1996; 271(40): 25027 - 25034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Buurman, K.-T. Kim, and W. Epstein Genetic Evidence for Two Sequentially Occupied K[IMAGE] Binding Sites in the Kdp Transport ATPase J. Biol. Chem., March 24, 1995; 270(12): 6678 - 6685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dorus, H. Mimura, and W. Epstein Substrate-binding Clusters of the K+-transporting Kdp ATPase of Escherichia coli Investigated by Amber Suppression Scanning Mutagenesis J. Biol. Chem., March 23, 2001; 276(13): 9590 - 9598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Senn, F. Rubio, M. A. Banuelos, and A. Rodriguez-Navarro Comparative Functional Features of Plant Potassium HvHAK1 and HvHAK2 Transporters J. Biol. Chem., November 21, 2001; 276(48): 44563 - 44569. [Abstract] [Full Text] [PDF]
|
|
|
|
