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The Journal of General Physiology, Vol 66, 357-382, Copyright © 1975 by The Rockefeller University Press
ARTICLES |
F Harosi
Absorption spectra and linear dichrosim of dark-adapted, isolated photoreceptors of mudpuppies, larval and adult tiger salamanders, and tropical toads were measured microspectrophotometrically. Spectral half- band width, dichroic ratio, and transverse specific density were determined using averaged polarized absorptance spectra and photomicrographs of seve types of rod outer segments. Ttwo classes of cells were found, one with higher specific density and dichroic ratio, associable with thr presence of rhodopsins, the other, lower in both quantities, associable with porphyropsins. Relationships were derived to calculate the product of molar concentration and extinction coefficient (Cepsilon max) from specific density and dichroic ratio. By utilizing the hypothesis of invariance of oscillator strengths and measured half-band widths, epsilon max values were independently determined, permitting the calculation of C. The pigment concentraation for all cells tested was about 3.5 mM. The broadness of green rod pigment spectra is correlated with reduced molar absorpitvity and reduced cellular specific density. Estimation of physiological spectral for the orientation of retinals in situ which could account for the apparent degree of aligment of transition moments. In the chosen orientation, the ring portion of conjugation becomes primarily responsible for axial extinction. Reduced dichroism of dehydroretinal- bearing cells can thus result from the extended ring conjugation of chromophores. Some inferences derivable from the model are discussed.
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