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The Journal of General Physiology, Vol 63, 389-414, Copyright © 1974 by The Rockefeller University Press

ARTICLE

Antibody-Induced Alterations in the Kinetic Characteristics of the Na:K Pump in Goat Red Blood Cells

John R. Sachs 1, J. Clive Ellory 1, Donna L. Kropp 1, Philip B. Dunham 1, and Joseph F. Hoffman 1

1 From the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.

Dr. Ellory's present address is the A. R. C. Institute of Animal Physiology, Babraham, Cambridge, England. Dr. Dunham's present address is the Department of Biology, Syracuse University, Syracuse, New York 13210.

The kinetic characteristics of the Na:K pump in high potassium (HK) and low potassium (LK) goat red cells were investigated after altering the intracellular cation concentrations. At low concentrations of intracellular K (Kc), increasing Kc at first stimulates the active K influx in HK cells, but at higher Kc the pump is inhibited. These results suggest that in HK cells Kc acts both at a stimulatory site at the inner aspect of the pump and by competition with intracellular Na (Nac) at the Na translocation sites. In LK cells, Kc inhibits the active K influx and the sensitivity of LK cells to inhibition is much greater than the sensitivity of HK cells. Exposure of LK cells to an antibody (anti-L), raised in an HK sheep by injection of LK sheep cells, increased the active K influx at any given Kc. The effect of the antibody was greater at higher intracellular K concentrations, and in cells with very low concentrations of K the antibody had little effect on the pump rate. The failure of anti-L to stimulate the pump in low Kc LK cells was not due to failure of the antibody to bind to the cells. Anti-L combining at the outer surface of the cell reduces the affinity of the pump at the inner surface for K at the inhibitory sites. The maximal pump rate in LK cells at optimal Na and K concentrations is less than the maximal pump rate of HK cells under the same circumstances.

Submitted on September 20, 1972


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