The Journal of General Physiology
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The Journal of General Physiology, Vol 63, 1-21, Copyright © 1974 by The Rockefeller University Press


ARTICLE

Linear Dichroism and Orientation of the Phycomyces Photopigment

A. J. Jesaitis 1

1 From the Division of Biology, California Institute of Technology, Pasadena, California 91109.

Dr. Jesaitis' present address is the Institut für Biologie III der Universität Freiburg, 78 Freiburg i. Br., Germany.

The greater sensitivity of a cylindrical Phycomyces sporangiophore to blue light polarized transversely rather than longitudinally is a consequence of the dichroism and orientation of the receptor pigment. The abilities of wild type and several carotene mutants to distinguish between the two directions of polarization are the same. The E-vector angle for maximum response relative to the transverse direction is 42 ± 4° at 280 nm, 7° ± 3° at 456 nm, and 7° ± 8° at 486 nm. The in vivo attenuation of polarized light at these wavelengths is very small. The polarized light effect in Phycomyces cannot arise from reflections at the cell surface or from differential attenuations due to internal screening or scattering.

Submitted on May 29, 1973


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[Abstract] [Full Text] [PDF]



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