The Journal of General Physiology
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The Journal of General Physiology, Vol 61, 290-304, Copyright © 1973 by The Rockefeller University Press

ARTICLE

Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues

Seiji Uehara 1

1 From the Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan

The epithelium of the circumvallate papillae of rat tongues was stripped off by treatment with 0.005% elastase in a state when many taste buds were present. The taste buds were isolated from the stripped epithelium by further treatment with 0.005% elastase and 0.08% trypsin. A protein which was thought to be characteristic of taste buds was found from semimicro disc polyacrylamide gel electrophoretic studies of the stripped epithelia with and without taste buds. This result was supported by micro disc polyacrylamide gel electrophoretic studies of isolated taste buds.

Submitted on July 24, 1972


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