The Journal of General Physiology
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The Journal of General Physiology, Vol 60, 388-405, Copyright © 1972 by The Rockefeller University Press

ARTICLE

Transport of Axonal Enzymes in Surviving Segments of Frog Sciatic Nerve

Lester M. Partlow 1, C. David Ross 1, Ratna Motwani 1, and David B. McDougal Jr. 1

1 From the Department of Pharmacology and Beaumont-May Institute of Neurology, Washington University Medical School, St. Louis, Missouri 63110.

Dr. Partlow's present address is the Department of Pharmacology, University of Utah, Salt Lake City, Utah 84112.

Redistribution of axonal enzymes as a function of time in vitro was studied in an unbranched segment of frog sciatic nerve. Cholinesterase activity moved peripherally at a rate of 99 mm/day and centrally at 19 mm/day. One-quarter of the total nerve content of the enzyme was estimated to be in motion, one-eighth in each direction. Mitochondrial enzymes (hexokinase and glutamic dehydrogenase) moved peripherally at 20–31 mm/day, centrally at 11–20 mm/day. Only 10% of the total content of these mitochondrial enzymes was in motion. No movement of choline acetylase or 6-phosphogluconic dehydrogenase activity was seen even after 4 days in vitro. However, in a 12 day in vivo experiment choline acetylase moved toward the periphery at a rate of 0.34 mm/day. After a day or so in vitro the distal accumulations of cholinesterase and glutamic dehydrogenase decreased, with a concomitant and quantitatively equivalent increase in enzyme activities at the proximal end of the nerve. It is postulated that during incubation a mechanism for reversing the direction of flow develops in the peripheral stump of the nerve. Vinblastine inhibited central and peripheral flow of both cholinesterase and glutamic dehydrogenase. Movement of cholinesterase was not affected by ouabain, thalidomide, or phenobarbital, nor by K+ excess (110 mM) or absence.

Submitted on March 15, 1972


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S. Younkin, R. Brett, B Davey, and L Younkin
Substances moved by axonal transport and released by nerve stimulation have an innervation-like effect on muscle
Science, June 16, 1978; 200(4347): 1292 - 1295.
[Abstract] [PDF]

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R Hammerschlag, A. Dravid, and A. Chiu
Mechanism of axonal transport: a proposed role for calcium ions
Science, April 18, 1975; 188(4185): 273 - 275.
[Abstract] [PDF]


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