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The Journal of General Physiology, Vol 58, 225-237, Copyright © 1971 by The Rockefeller University Press

ARTICLE

Characterization and Analysis of Frog Photoreceptor Membranes

Deric Bownds 1, Ann Gordon-Walker 1, Anne-Claude Gaide-Huguenin 1, and William Robinson 1

1 From the Laboratory of Molecular Biology and the Department of Zoology, University of Wisconsin, Madison, Wisconsin 53706

Frog photoreceptor membranes contain 54,000 g of protein per mole of visual pigment chromophore, virtually all of it insoluble membrane protein. Acrylamide gel electrophoresis indicates one major polypeptide class, most likely the visual pigment apoprotein. Suspensions of these photoreceptor membranes accumulate calcium ions when ATP is present, a characteristic that may play a part in visual excitation.

Submitted on January 23, 1971


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