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The Journal of General Physiology, Vol 49, 717-725, Copyright © 1966 by The Rockefeller University Press

ARTICLE

Cellular Electrophoretic Mobility and the Mitotic Cycle

E. Mayhew 1

1 From the Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo

The electrophoretic mobility of RPMI No. 41 cells grown in suspension, parasynchronized by double thymidine blocking and cold shock, is reported. No. 41 cells have a higher electrophoretic mobility during the mitotic peak phase than at other times in the mitotic cycle. Treatment of parasynchronous cells by neuraminidase reduces the mobility to the same value irrespective of the stage of the cells in the mitotic cycle. The higher electrophoretic mobility of cells in mitotic peak phase is probably due to a higher surface charge density at this time, possibly caused by a higher concentration of ionized neuraminic acid carboxyl groups at the hydrodynamic shear layer. The mobility of nonsynchronous rapidly and slowly growing cells differs; neuraminidase reduces their mobility by proportionately similar amounts. The results suggest that the differences in mobility between rapidly and slowly growing cells cannot be accounted for exclusively by differences in the amount of neuraminic acid groups at the shear layer.

Submitted on July 20, 1965


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L. L. Hause, R. A. Pattillo, A. Sances Jr., and R. F. Mattingly
Cell Surface Coatings and Membrane Potentials of Malignant and Nonmalignant Cells
Science, August 7, 1970; 169(3945): 601 - 603.
[Abstract] [PDF]


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