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The Journal of General Physiology, Vol 38, 169-179, Copyright © 1954 by The Rockefeller University Press

ARTICLE

REVERSIBLE INACTIVATION OF TYPHUS RICKETTSIAE

I. INACTIVATION BY FREEZING



Marianna R. Bovarnick 1 and Emma G. Allen 1

1 From the Laboratory Service, Veterans Administration Hospital, Brooklyn, New York, and the Department of Medicine, State University of New York at New York City

Rickettsiae that have been frozen and thawed in isotonic salt solutions show greatly decreased toxicity for mice, hemolytic activity, respiration, and infectivity for eggs. All these properties can be partially restored by incubation of the rickettsiae in the presence of DPN and coenzyme A for 2 hours at 34°C.

The extent of both inactivation and of subsequent reactivation is markedly affected by the presence of low concentrations of sucrose during the process of freezing and thawing.

It has been shown that DPN is present in rickettsial suspensions and that in preparations that have not been frozen, the DPN sediments with the rickettsiae. After freezing in isotonic salt solution the DPN becomes non-sedimentable.

Submitted on June 9, 1954


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