The Journal of General Physiology
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The Journal of General Physiology, Vol 32, 265-269, Copyright © 1948 by The Rockefeller University Press

ARTICLE

CRYSTALLIZATION OF SALT-FREE CHYMOTRYPSINOGEN AND CHYMOTRYPSIN FROM SOLUTION IN DILUTE ETHYL ALCOHOL

M. Kunitz 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey

Chymotrypsinogen and chymotrypsin crystallize readily from dilute solutions of ethyl alcohol in the absence of salts. The crystals formed in the presence of alcohol differ in appearance from those formed in the presence of ammonium sulfate. Chymotrypsinogen yields well formed polyhedrons instead of fine needles usually produced in ammonium sulfate solution. Chymotrypsin yields fine needles in the presence of alcohol and rhombohedrons in the presence of ammonium sulfate. The enzymatic properties of the crystals formed in the presence of alcohol are identical with those of the crystals isolated in the presence of ammonium sulfate.

Submitted on June 21, 1948


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