The Journal of General Physiology
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The Journal of General Physiology, Vol 28, 449-461, Copyright © 1945 by The Rockefeller University Press

ARTICLE

METABOLISM OF TISSUE CULTURES

III. A METHOD FOR MEASURING THE PERMEABILITY OF TISSUE CELLS TO SOLUTES



Waldo E. Cohn 1 and Austin M. Brues 1

1 From the Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University at the Massachusetts General Hospital, Boston

By using radioactive isotopes in tissue cultures, the rate of permeation of substances into cells can be measured independently of concurrent metabolic reactions of these substances. Techniques of obtaining and analyzing data are described. Examples are given using radioactive potassium and phosphorus.

Using cultures of chick embryo muscle, turnover time for cell potassium is 6 hours, and for cell inorganic phosphate is 7 hours in the examples cited. Permeability rates, based on estimates of the cell surface involved and expressed as millimoles per cm.2 per hour, are of the order of magnitude of 10–6 for potassium and of 10–7 for phosphate.

Submitted on December 14, 1944


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