The Journal of General Physiology
Axon Instruments microelectrode amplifiers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 455K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carr, C. W.
Right arrow Articles by Sollner, K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Carr, C. W.
Right arrow Articles by Sollner, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 28, 179-185, Copyright © 1945 by The Rockefeller University Press

ARTICLE

THE STRUCTURE OF THE COLLODION MEMBRANE AND ITS ELECTRICAL BEHAVIOR

XII. THE PREPARATION AND PROPERTIES OF "MEGAPERMSELECTIVE" PROTAMINE COLLODION MEMBRANES COMBINING HIGH IONIC SELECTIVITY WITH HIGH PERMEABILITY



Charles W. Carr 1, Harry P. Gregor 1, and Karl Sollner 1

1 From the Department of Physiology, University of Minnesota, Minneapolis

The technique of Abrams and Sollner for the preparation of electropositive dried protamine collodion membranes has been improved. Porous collodion membranes cast on the outside of rotating tubes are treated for 48 hours with a solution of 2 per cent protamine sulfate buffered at pH 11. After being washed thoroughly the membranes are dried in air for several hours, soaked in water for several hours, and removed from the tubes. Further drying in air but without support shrinks the membranes slightly. The resulting membranes are designated "permselective" or "megapermselective" protamine collodion membranes. These membranes regularly give characteristic concentration potentials of –52 to –53 mv. and (in 0.1 M KCl) resistance of 0.5 to 15 ohms per membrane of 50 cm.2 area. This resistance is several orders of magnitude smaller than that of the conventional dyestuff- and alkaloid-impregnated positive membranes. The megapermselective protamine collodion membranes can be kept either dry or in water for prolonged periods without detectable deterioration. They are quite smooth, have a regular shape, and stand considerable handling without breakage.

The megapermselective protamine collodion membranes are the electropositive analogues of the electronegative megapermselective collodion membranes described by Carr and Sollner.

Submitted on September 5, 1944


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents