The Journal of General Physiology
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The Journal of General Physiology, Vol 18, 485-490, Copyright © 1935 by The Rockefeller University Press


ARTICLE

FREEZING POINTS OF ANTI-COAGULANT SALT SOLUTIONS

David I. Hitchcock 1 and Ruth B. Dougan 1

1 From the Laboratory of Physiology, Yale University School of Medicine, New Haven

By a method involving equilibration of ice and solution, and analysis of the solution, freezing point depressions of solutions of sodium citrate, oxalate, and fluoride have been determined over the range Delta = 0.45 to 0.65°C. Determinations with sodium chloride solutions have confirmed the accuracy of the method. In each case the freezing point depression is given, within 0.002°C., as a linear function of the concentration. By the use of these linear equations it is possible to prepare a solution of any of these four salts isotonic with a given biological fluid of known freezing point, provided the latter falls within the range studied.

Accepted on June 20, 1934


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