The Journal of General Physiology
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The Journal of General Physiology, Vol 18, 459-466, Copyright © 1935 by The Rockefeller University Press

ARTICLE

A METHOD FOR DETERMINING THE RENNET ACTIVITY OF CHYMO-TRYPSIN

M. Kunitz 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.

The rennet activity of chymo-trypsin (or pepsin) is conveniently measured by allowing a standard solution of milk to which chymotrypsin has been added to flow slowly through a graduated pipette and observing the rate and distance of flow of the milk before it clots. The time required for chymo-trypsin to clot milk may be calculated from these observations. The rennet activity is expressed as the reciprocal of the time in minutes required for 1 ml. of enzyme solution to clot 10 ml. of standard milk powder solution.

Accepted on May 28, 1934


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