I. METHODS OF ESTIMATION

A. E. Mirsky ¹ and M. L. Anson ¹

¹ From the Hospital of The Rockefeller Institute for Medical Research, New York, and the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.

1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups.

2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present.

3. A method for estimating the cysteine content of proteins has been described.

4. With these methods, estimations have been made of the S-S and SH groups and of the cystine and cysteine contents of a number of proteins.

5. In a denatured, but unhydrolyzed protein, the number of S-S and SH groups is equivalent to the quantity of cystine and cysteine found in the protein after hydrolysis.

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