The Journal of General Physiology
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The Journal of General Physiology, Vol 18, 143-162, Copyright © 1934 by The Rockefeller University Press

ARTICLE

THE PREPARATION OF THE GRADED COLLODION MEMBRANES OF ELFORD AND THEIR USE IN THE STUDY OF FILTERABLE VIRUSES

Johannes H. Bauer 1 and Thomas P. Hughes 1

1 From the Laboratories of the International Health Division of the Rockefeller Foundation, New York

1. The method described by Elford for the preparation of graded collodion membranes suitable for ultrafiltration was found to give excellent results, and his findings are fully confirmed.

2. A formula is given for the preparation of collodion from which satisfactory membranes of graded porosity can be prepared.

3. The technique and apparatus used in the preparation, and standardization of membranes are described in detail.

4. The technique and apparatus required for ultrafiltration experiments are described, and some drawbacks encountered in the experiments are discussed.

5. The results of ultrafiltration experiments show that the pores of the membranes are remarkably uniform in size.

Accepted on April 18, 1934


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