The Journal of General Physiology, Vol 17, 151-157,
Copyright © 1933 by The Rockefeller University Press
THE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN
M. L. Anson 1 and
A. E. Mirsky 1
1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller Institute for Medical Research, New York
The formation from hemoglobin of split products not precipitable by trichloracetic acid is taken as a measure of tryptic activity. The split products are estimated colorimetrically.
Many measurements of tryptic activity can be made in a short time and different samples of hemoglobin yield the same results.
Accepted on August 10, 1933