The Journal of General Physiology
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The Journal of General Physiology, Vol 16, 375-395, Copyright © 1932 by The Rockefeller University Press

ARTICLE

BIOELECTRIC POTENTIALS IN VALONIA

THE EFFECT OF SUBSTITUTING KCl FOR NaCl IN ARTIFICIAL SEA WATER



E. B. Damon 1

1 From the Laboratories of The Rockefeller Institute for Medical Research

The P.D. across the protoplasm of Valonia macrophysa has been studied while the cells were exposed to artificial solutions resembling sea water in which the concentration of KCl was varied from 0 to 0.500 mol per liter. The P.D. across the protoplasm is decreased by lowering and increased by raising the concentration of KCl in the external solution. Changes in P.D. with time when the cell is treated with KCl-rich sea water resemble those observed with cells exposed to Valonia sap.

Varying the reaction of natural sea water from pH 5 to pH 10 has no appreciable effect on the P.D. across Valonia protoplasm. Similarly, varying the pH of KCl-rich sea water within these limits does not alter the height of the first maximum in the P.D.-time curve. The subsequent behavior of the P.D., however, is considerably affected by the pH of the KCl-rich sea water. These changes in the shape of the P.D.-time curve have been interpreted as indicating that potassium enters Valonia protoplasm more rapidly from alkaline than from acidified KCl-rich sea water. This conclusion is discussed in relation to certain theories which have been proposed to explain the accumulation of KCl in Valonia sap.

The initial rise in P.D. when a Valonia cell is transferred from natural sea water to KCl-rich sea water has been correlated with the concentrations of KCl in the sea waters. It is assumed that the observed P.D. change represents a diffusion potential in the external surface layer of the protoplasm, where the relative mobilities of ions may be supposed to differ greatly from their values in water. Starting with either Planck's or Henderson's formula, an equation has been derived which expresses satisfactorily the observed relationship between P.D. change and concentration of KCl. The constants of this equation are interpreted as the relative mobilities of K+, Na+, and Cl- in the outer surface layer of the protoplasm. The apparent relative mobility of K+ has been calculated by inserting in this equation the values for the relative mobilities of Na+ (0.20) and Cl- (1.00) determined from earlier measurements of concentration effect with natural sea water. The average value for the relative mobility of K+ is found to be about 20. The relative mobility may vary considerably among different individual cells, and sometimes also in the same individual under different conditions.

Calculation of the observed P.D. changes as phase-boundary potentials proved unsatisfactory.

Accepted on July 29, 1932


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