The Journal of General Physiology
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The Journal of General Physiology, Vol 16, 33-40, Copyright © 1932 by The Rockefeller University Press


ARTICLE

CRYSTALLINE PEPSIN

IV. HYDROLYSIS AND INACTIVATION BY ACID



John H. Northrop 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N.J.

The decrease in protein nitrogen and in the activity of solutions of crystalline pepsin at pH 1.8 and 45°C. has been determined. The decrease in activity, as measured with eleven different methods, is in exact proportion to the decrease of protein nitrogen of the solution. The measurements were continued until less than 5 per cent of the original protein remained. These results indicate that none of the split products of the protein molecule possess any appreciable activity compared to that of the original protein.

Accepted on May 20, 1932


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