The Journal of General Physiology
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The Journal of General Physiology, Vol 14, 605-609, Copyright © 1931 by The Rockefeller University Press


ARTICLE

PROTEIN COAGULATION AND ITS REVERSAL

GLOBIN



M. L. Anson 1 and A. E. Mirsky 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller Institute for Medical Research, New York

1. The globin prepared from hemoglobin by the acid acetone method is denatured globin.

2. The denaturation and coagulation of globin by acid acetone are reversible.

3. Soluble globin can be obtained from the acid acetone globin even if the globin is first precipitated by trichloracetic acid or heated to 100°C.

4. Hill and Holden's theory that they separated native globin from hemoglobin without any intermediate denaturation is not proven by their experiments.

Accepted on March 30, 1931


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