The Journal of General Physiology
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The Journal of General Physiology, Vol 14, 547-562, Copyright © 1931 by The Rockefeller University Press

ARTICLE

DETACHMENT OF BACTERIOPHAGE FROM ITS CARRIER PARTICLES

D. M. Hetler 1 and J. Bronfenbrenner 1

1 From the Department of Bacteriology and Immunology, Washington University Medical School, St. Louis

The active substance (phage) present in the lytic broth filtrate is distributed through the medium in the form of particles. These particles vary in size within broad limits. The average size of these particles as calculated on the basis of the rate of diffusion approximates 4.4 mµ in radius. Fractionation by means of ultrafiltration permits partial separation of particles of different sizes. Under conditions of experiments here reported the particles varied in the radius size from 0.6 mµ to 11.4 mµ. The active agent apparently is not intimately identified with these particles. It is merely carried by them by adsorption, and under suitable experimental conditions it can be detached from the larger particles and redistributed on smaller particles of the medium.

Accepted on February 3, 1931


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F. M. EXNER and S. E. LURIA
THE SIZE OF STREPTOCOCCUS BACTERIO-PHAGES AS DETERMINED BY X-RAY INACTIVATION
Science, October 24, 1941; 94(2443): 394 - 395.
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