The Journal of General Physiology
CrossRef
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1098K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krueger, A. P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Krueger, A. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 14, 493-516, Copyright © 1931 by The Rockefeller University Press

ARTICLE

THE SORPTION OF BACTERIOPHAGE BY LIVING AND DEAD SUSCEPTIBLE BACTERIA

I. EQUILIBRIUM CONDITIONS



Albert P. Krueger 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.

The above data relating to the antistaphylococcus phage and single strain of S. aureus with which previous papers have been concerned (9, 10, 11, 12), bring out the following points.

(a) For live, resting, susceptible B suspended in broth as well as for B in a P-B mixture during the logarithmic phases of B growth and P formation, P is distributed in a manner typical of numerous materials soluble in both phases of a two phase system, i.e., distribution proceeds in accordance with the equation Cb/Ca = K where Cb = extracellular P/ml. of broth and Ca = intracellular P/ml. of B. The combination is quantitatively reversible.

(b) With heat-killed, susceptible B, P distribution is of the adsorptive type, expressible in the form of the adsorption isotherm equation a = kC1/n. The average value of 1/n is 0.80 in agreement with the results of von Angerer (2). Under ordinary conditions dead B take up much more P than do live B, the reaction proceeding to > 99 per cent completion. The combination of P with dead B is not demonstrably reversible and with high initial P/B ratios saturation of B with P is effected. Bacteria killed at 65°C., 80°C. and 100°C. show no differences in adsorptive ability.

(c) The rates at which live, resting, susceptible B and heat-killed, susceptible B remove P from solution do not differ significantly. Velocity constants of the process calculated from

See PDF for Equation

agree satisfactorily. It is shown that the time interval consumed is concerned with an actual reaction between P and B and not with diffusion of P through the broth to B.

(d) P determinations have been found to serve as satisfactory indicators for B growth in P-B mixtures where [B] is to be maintained at a constant level. Very small increments in [B] give rise to measurable increases in P by virtue of the fact that dP/dt is proportional to a power of the rate dB/dt (9).

(e) Similarly [P] estimations will detect death of B cells in P-live B suspensions. Dead B take up large amounts of P irreversibly; such P cannot function in the titration and the result is a sharp drop in [P] of controls.

Accepted on January 20, 1931


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
P. Mudgal, F. Breidt Jr., S. R. Lubkin, and K. P. Sandeep
Quantifying the significance of phage attack on starter cultures: a mechanistic model for population dynamics of phage and their hosts isolated from fermenting sauerkraut.
Appl. Envir. Microbiol., June 1, 2006; 72(6): 3908 - 3915.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents