|
|
|
|
|
|
|
||
ARTICLE |
Correspondence to Mark O. Bevensee: bevensee{at}physiology.uab.edu
Using pH- and voltage-sensitive microelectrodes, as well as the two-electrode voltage-clamp and macropatch techniques, we compared the functional properties of the three NBCe1 variants (NBCe1-A, -B, and -C) with different amino and/or carboxy termini expressed in Xenopus laevis oocytes. Oocytes expressing rat brain NBCe1-B and exposed to a CO2/HCO3– solution displayed all the hallmarks of an electrogenic Na+/HCO3– cotransporter: (a) a DIDS-sensitive pHi recovery following the initial CO2-induced acidification, (b) an instantaneous hyperpolarization, and (c) an instantaneous Na+-dependent outward current under voltage-clamp conditions (–60 mV). All three variants had similar external HCO3– dependencies (apparent KM of 4–6 mM) and external Na+ dependencies (apparent KM of 21–36 mM), as well as similar voltage dependencies. However, voltage-clamped oocytes (–60 mV) expressing NBCe1-A exhibited peak HCO3–-stimulated NBC currents that were 4.3-fold larger than the currents seen in oocytes expressing the most dissimilar C variant. Larger NBCe1-A currents were also observed in current–voltage relationships. Plasma membrane expression levels as assessed by single oocyte chemiluminescence with hemagglutinin-tagged NBCs were similar for the three variants. In whole-cell experiments (Vm = –60 mV), removing the unique amino terminus of NBCe1-A reduced the mean HCO3–-induced NBC current 55%, whereas removing the different amino terminus of NBCe1-C increased the mean NBC current 2.7-fold. A similar pattern was observed in macropatch experiments. Thus, the unique amino terminus of NBCe1-A stimulates transporter activity, whereas the different amino terminus of the B and C variants inhibits activity. One or more cytosolic factors may also contribute to NBCe1 activity based on discrepancies between macropatch and whole-cell currents. While the amino termini influence transporter function, the carboxy termini influence plasma membrane expression. Removing the entire cytosolic carboxy terminus of NBCe1-C, or the different carboxy terminus of the A/B variants, causes a loss of NBC activity due to low expression at the plasma membrane.
Abbreviations used in this paper: AE, anion exchanger; DIDS, 4,4'-diisothiocyanatostilbene-2,2' disulfonate; HA, hemagglutinin; HRP, horseradish peroxidase; NBC, Na/bicarbonate cotransporter; pHi, intracellular pH; PIP2, phosphatidylinositol 4,5-bisphosphate; SOC, single-oocyte chemiluminescence.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. D. Parker, P. Bouyer, C. M. Daly, and W. F. Boron Cloning and characterization of novel human SLC4A8 gene products encoding Na+-driven Cl-/HCO3- exchanger variants NDCBE-A, -C, and -D Physiol Genomics, August 1, 2008; 34(3): 265 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Chang, J. DiPiero, F. D. Sonnichsen, and M. F. Romero Entry to "Formula Tunnel" Revealed by SLC4A4 Human Mutation and Structural Model J. Biol. Chem., June 27, 2008; 283(26): 18402 - 18410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Choi, H. Soo Yang, and W. F. Boron The electrogenicity of the rat sodium-bicarbonate cotransporter NBCe1 requires interactions among transmembrane segments of the transporter J. Physiol., January 1, 2007; 578(1): 131 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. McAlear and M. O. Bevensee A Cysteine-scanning Mutagenesis Study of Transmembrane Domain 8 of the Electrogenic Sodium/Bicarbonate Cotransporter NBCe1 J. Biol. Chem., October 27, 2006; 281(43): 32417 - 32427. [Abstract] [Full Text] [PDF]
|
|
|
|
