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Published online Aug 29 2005. doi:10.1085/jgp.200509293
The Rockefeller University Press, 0022-1295 $8.00
JGP, Volume 126, Number 3, 205-212
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ARTICLE

 Localization of the Activation Gate of a Voltage-gated Ca2+ Channel

Cheng Xie, Xiao-guang Zhen, and Jian Yang

Department of Biological Sciences, Columbia University, New York, NY 10027

Correspondence to Jian Yang: jy160{at}columbia.edu

Ion channels open and close in response to changes in transmembrane voltage or ligand concentration. Recent studies show that K+ channels possess two gates, one at the intracellular end of the pore and the other at the selectivity filter. In this study we determined the location of the activation gate in a voltage-gated Ca2+ channel (VGCC) by examining the open/closed state dependence of the rate of modification by intracellular methanethiosulfonate ethyltrimethylammonium (MTSET) of pore-lining cysteines engineered in the S6 segments of the {alpha}1 subunit of P/Q type Ca2+ channels. We found that positions above the putative membrane/cytoplasm interface, including two positions below the corresponding S6 bundle crossing in K+ channels, showed pronounced state-dependent accessibility to internal MTSET, reacting ~1,000-fold faster with MTSET in the open state than in the closed state. In contrast, a position at or below the putative membrane/cytoplasm interface was modified equally rapidly in both the open and closed states. Our results suggest that the S6 helices of the {alpha}1 subunit of VGCCs undergo conformation changes during gating and the activation gate is located at the intracellular end of the pore.

C. Xie and X.-g. Zhen contributed equally to this work.

Abbreviations used in this paper: CNG, cyclic nucleotide–gated; MTSET, methanethiosulfonate ethyltrimethylammonium; VGCC, voltage-gated Ca2+ channel.


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Correction 126, p. 205
Cheng Xie, Xiao-guang Zhen, and Jian Yang
J. Gen. Physiol. 2006 127: 768. [Full Text] [PDF]



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