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J. Gen. Physiol.,
Volume 112, Number 1, July 1, 1998 85-93
From the * Max-Planck Society, Research Unit Molecular and Cellular Biophysics, D-07747 Jena, Germany; C-type inactivation in Shaker potassium channels inhibits K+ permeation. The associated structural
changes appear to involve the outer region of the pore. Recently, we have shown that C-type inactivation involves
a change in the selectivity of the Shaker channel, such that C-type inactivated channels show maintained voltage-sensitive activation and deactivation of Na+ and Li+ currents in K+-free solutions, although they show no measurable ionic currents in physiological solutions. In addition, it appears that the effective block of ion conduction
produced by the mutation W434F in the pore region may be associated with permanent C-type inactivation of
W434F channels. These conclusions predict that permanently C-type inactivated W434F channels would also show
Na+ and Li+ currents (in K+-free solutions) with kinetics similar to those seen in C-type-inactivated Shaker channels. This paper confirms that prediction and demonstrates that activation and deactivation parameters for this
mutant can be obtained from macroscopic ionic current measurements. We also show that the prolonged Na+ tail
currents typical of C-type inactivated channels involve an equivalent prolongation of the return of gating charge,
thus demonstrating that the kinetics of gating charge return in W434F channels can be markedly altered by
changes in ionic conditions.
Békésy Laboratory of Neurobiology,
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