|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 108, 157-175, Copyright © 1996 by The Rockefeller University Press
ARTICLES |
HC Hartzell
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030, USA. criss@anatomy.emory.edu
Xenopus oocytes are an excellent model system for studying Ca signaling. The purpose of this study was to characterize in detail the Ca-activated Cl currents evoked by injection of inositol 1,4,5- trisphosphate (IP3) into Xenopus oocytes voltage-clamped with two microelectrodes. Injection of IP3 into Xenopus oocytes activates two different Ca-activated Cl currents. ICl-1 is stimulated rapidly (within 5 s after IP3 injection), exhibits time-dependent activation upon depolarization, a linear instantaneous IV relationship with a reversal potential near ECl, and a curvilinear activation curve with an approximate half-maximal activation voltage of > 200 mV. ICl-2D is stimulated slowly after IP3 injection (half-maximal stimulation occurs approximately 3 min after injection). ICl-2D has a strongly outwardly rectifying instantaneous IV relationship with a reversal potential near ECl and is activated by hyperpolarization with a half-maximal activation voltage of -105 mV. ICl-2D cannot be activated by Ca released from stores but is activated by Ca influx. In contrast, ICl-1 can be stimulated by Ca released from intracellular Ca stores. It can also be stimulated by Ca influx through store-operated channels if the Ca driving force is increased by a hyperpolarization immediately before the depolarization that gates ICl-1 channels. The description of two currents activated by influx and Ca release from stores provides new insights into and questions about the regulation of Ca in Xenopus oocytes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. N. Chernova, D. H. Vandorpe, J. S. Clark, J. I. Williams, M. A. Zasloff, L. Jiang, and S. L. Alper Apparent receptor-mediated activation of Ca2+-dependent conductive Cl- transport by shark-derived polyaminosterols Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1644 - R1658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Andre, H. Boukhaddaoui, B. Campo, M. Al-Jumaily, V. Mayeux, D. Greuet, J. Valmier, and F. Scamps Axotomy-Induced Expression of Calcium-Activated Chloride Current in Subpopulations of Mouse Dorsal Root Ganglion Neurons J Neurophysiol, December 1, 2003; 90(6): 3764 - 3773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Machaca Ca2+-Calmodulin-dependent Protein Kinase II Potentiates Store-operated Ca2+ Current J. Biol. Chem., September 5, 2003; 278(36): 33730 - 33737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Mischel, J. A. Umbach, S. Eskandari, S. G. Smith, C. B. Gundersen, and G. A. Zampighi Nerve Growth Factor Signals via Preexisting TrkA Receptor Oligomers Biophys. J., August 1, 2002; 83(2): 968 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kroslak, T. Koch, E. Kahl, and V. Hollt Human Phosphatidylethanolamine-binding Protein Facilitates Heterotrimeric G Protein-dependent Signaling J. Biol. Chem., October 19, 2001; 276(43): 39772 - 39778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. P. Hamill and B. Martinac Molecular Basis of Mechanotransduction in Living Cells Physiol Rev, April 1, 2001; 81(2): 685 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kuruma, Y. Hirayama, and H. C. Hartzell A hyperpolarization- and acid-activated nonselective cation current in Xenopus oocytes Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1401 - C1413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Callamaras and I. Parker Ca2+-dependent activation of Cl- currents in Xenopus oocytes is modulated by voltage Am J Physiol Cell Physiol, April 1, 2000; 278(4): C667 - C675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Chiu, L. Ross, B. Cohen, H. Lester, and S. Gill The transporter-like protein inebriated mediates hyperosmotic stimuli through intracellular signaling J. Exp. Biol., January 12, 2000; 203(23): 3531 - 3546. [Abstract] [PDF]
|
|
|
|
|
|
A. Takahashi, P. Camacho, J. D. Lechleiter, and B. Herman Measurement of Intracellular Calcium Physiol Rev, October 1, 1999; 79(4): 1089 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Machaca and H. C. Hartzell Adenophostin A and Inositol 1,4,5-Trisphosphate Differentially Activate Cl- Currents in Xenopus Oocytes Because of Disparate Ca2+ Release Kinetics J. Biol. Chem., February 19, 1999; 274(8): 4824 - 4831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuruma and H. C. Hartzell Dynamics of calcium regulation of chloride currents in Xenopus oocytes Am J Physiol Cell Physiol, January 1, 1999; 276(1): C161 - C175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Shuttleworth and J. L. Thompson Muscarinic Receptor Activation of Arachidonate-mediated Ca2+ Entry in HEK293 Cells Is Independent of Phospholipase C J. Biol. Chem., December 4, 1998; 273(49): 32636 - 32643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Oz, M. T. Melia, N. M. Soldatov, D. R. Abernethy, and M. Morad Functional Coupling of Human L-Type Ca2+ Channels and Angiotensin AT1A Receptors Coexpressed in Xenopus laevis Oocytes: Involvement of the Carboxyl-Terminal Ca2+ Sensors Mol. Pharmacol., December 1, 1998; 54(6): 1106 - 1112. [Abstract] [Full Text]
|
|
|
|
|
|
D. H. Vandorpe, B. E. Shmukler, L. Jiang, B. Lim, J. Maylie, J. P. Adelman, L. de Franceschi, M. D. Cappellini, C. Brugnara, and S. L. Alper cDNA Cloning and Functional Characterization of the Mouse Ca2+-gated K+ Channel, mIK1. ROLES IN REGULATORY VOLUME DECREASE AND ERYTHROID DIFFERENTIATION J. Biol. Chem., August 21, 1998; 273(34): 21542 - 21553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Hartzell, K. Machaca, and Y. Hirayama Effects of Adenophostin-A and Inositol-1,4,5-trisphosphate on Cl- Currents in Xenopus laevis Oocytes Mol. Pharmacol., April 1, 1997; 51(4): 683 - 692. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. K. Silverman, P. Kofuji, D. A. Dougherty, N. Davidson, and H. A. Lester A regenerative link in the ionic fluxes through the weaver potassium channel underlies the pathophysiology of the mutation PNAS, December 24, 1996; 93(26): 15429 - 15434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Xu, F. J. Longo, M. R. Wintermantel, X. Jiang, R. A. Clark, and S. DeLisle Calreticulin Modulates Capacitative Ca2+ Influx by Controlling the Extent of Inositol 1,4,5-Trisphosphate-induced Ca2+ Store Depletion J. Biol. Chem., November 17, 2000; 275(47): 36676 - 36682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Mischel, S. G. Smith, E. R. Vining, J. S. Valletta, W. C. Mobley, and L. F. Reichardt The Extracellular Domain of p75NTR Is Necessary to Inhibit Neurotrophin-3 Signaling through TrkA J. Biol. Chem., March 30, 2001; 276(14): 11294 - 11301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Machaca and S. Haun Store-operated Calcium Entry Inactivates at the Germinal Vesicle Breakdown Stage of Xenopus Meiosis J. Biol. Chem., December 1, 2000; 275(49): 38710 - 38715. [Abstract] [Full Text] [PDF]
|
|
|
|
